Primer designing tool - National Center for Biotechnology ...
WEBPrimer-blast tries to find target-specific primers by placing candidate primers on unique template regions that are not similar to other targets.
PCR primer design - QIAGEN
WEBOptimal primer sequences and appropriate primer concentrations are essential for maximal specificity and efficiency in PCR. The table, Primer design and usage guidelines, provides an overview of primer design and use for standard and …
Addgene: Protocol - How to Design Primers
WEBOne needs to design primers that are complementary to the template region of DNA. They are synthesized chemically by joining nucleotides together. One must selectively block and unblock repeatedly the reactive groups on a nucleotide when adding a …
NEB ® Primer Design Tools
WEBWhether you are creating an assembly that incorporates multiple fragments, conducting your entire PCR at a single temperature, or incorporating intentionally mismatched nucleotides, careful primer design can set you up for …
Primer3
WEBPrimer3 is a program for designing PCR primers (PCR = "Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in genetics and molecular biology. Primer3 can also design hybridization probes and sequencing primers.
PCR Primer Design Tips - Behind the Bench
WEBSep 25, 2019 · Here are some guidelines for designing your PCR primers: Aim for the GC content to be between 40 and 60% with the 3’ of a primer ending in G or C to promote binding. This is known as a GC Clamp. The G and C bases have stronger hydrogen bonding and help with the stability of the primer.
Design PCR primers and check them for specificity
WEBEnter the target sequence in FASTA format or an accession number of an NCBI nucleotide sequence in the PCR Template section of the form. If the NCBI mRNA reference sequence accession number is used, the tool will automatically design primers that are specific to that splice variant.