Circular RNAs as microRNA sponges: evidence and controversies
WEBOct 27, 2021 · The circRNAs constitute a large class of mainly non-coding RNAs, which have been extensively studied in recent years, in particular in the cancer research field where many circRNAs have been proposed to function as miR sponges. Here, we briefly describe miR-mediated gene regulation and the extra …
The biogenesis, biology and characterization of circular RNAs
WEBAug 8, 2019 · Circular RNAs (circRNAs) are covalently closed, endogenous biomolecules in eukaryotes with tissue-specific and cell-specific expression patterns, whose biogenesis is regulated by specific cis...
Natural RNA circles function as efficient microRNA sponges
WEBFeb 27, 2013 · Here we show that this circRNA acts as a miR-7 sponge; we term this circular transcript ciRS-7 (circular RNA sponge for miR-7). ciRS-7 contains more than 70 selectively conserved miRNA target...
Circular RNAs Act as miRNA Sponges - PubMed
WEBCircRNAs can regulate gene expression by influencing the transcription, the mRNA turnover, and translation by sponging RNA-binding proteins and microRNAs. Given the broad impact of circRNA on miRNA activity, there is huge interest in understanding the impact of miRNA sponging by circRNA on gene regulation.
Specific expression and functions of circular RNAs
WEBFeb 15, 2022 · In recent years, circular RNAs (circRNAs), a new class of RNA molecules characterized by their covalently closed circular structure, have become a new research paradigm in RNA biology.
Natural RNA circles function as efficient microRNA sponges
WEBMar 21, 2013 · Here we show that this circRNA acts as a miR-7 sponge; we term this circular transcript ciRS-7 (circular RNA sponge for miR-7). ciRS-7 contains more than 70 selectively conserved miRNA target sites, and it is highly and widely associated with Argonaute (AGO) proteins in a miR-7-dependent manner.
Circular RNAs: Characterization, cellular roles, and applications
WEBMay 17, 2022 · Circular RNA sponge containing four miRNA-122 binding sites, synthesized using 5′-3′ end-ligation by T4 RNA ligase 1 (Figure 1K), sequestered miRNA-122, thereby inhibiting viral protein production with a higher efficiency than Miravirsen (Jost et al., 2018).
Circular RNA-protein interactions: functions, mechanisms, and ...
WEBFeb 10, 2020 · Different RNA enzymes have corresponding RNA substrates; thus, the target RNA can be purified continuously allowing the target circRNAs to be obtained. CircRNAs can be enriched by excluding rRNA, tRNA, poly(A) + RNAs, and linear RNAs with the use of their corresponding RNA enzymes or with PCR.
Circular RNAs: Promising Biomarkers for Human Diseases
WEBJun 6, 2018 · Recent studies have suggested that circRNAs play a crucial role in regulating gene expression by acting as a microRNA sponge, RNA binding protein sponge and translational regulator. CircRNAs have become a research hotspot because of their close association with the development of diseases.
Artificial Circular RNA Sponges Targeting MicroRNAs as a …
WEBSep 6, 2019 · Synthetic Circular RNA Functions as a miR-21 Sponge to Suppress Gastric Carcinoma Cell Proliferation