Plasmids 101: Gateway Cloning - Addgene
WEBYou can use Gateway cloning to insert multiple DNA fragments into many vectors at once in the same tube. You can clone up to 4 DNA fragments, in a specific order and orientation, in one tube, into one Gateway vector to produce the desired expression clone. This is possible thanks to the Gateway vectors’ design.
Gateway Cloning Technique - SnapGene
WEBWhat is Gateway cloning? Gateway cloning is a highly efficient alternative to restriction cloning and does not require the use of restriction enzymes. Instead, an insert is moved into a vector through a two-step recombination process that takes advantage of integration and excision reactions using attachment sites attL and attB.
Gateway Cloning | Thermo Fisher Scientific - US
WEBExperience Gateway cloning technology. Fast reactions—1 hour room-temperature cloning reactions; Accurate results—cloning reactions achieve >95% efficiency to deliver the clone you need; Versatile technology—easily shuttle DNA material/ insert from vector to vector
Gateway Recombination Cloning Technology | Thermo Fisher …
WEBGateway technology facilitates cloning of genes, into and back out of, multiple vectors via site-specific recombination. Once a gene is cloned into an Entry clone you can then move the DNA fragment into one or more destination vectors simultaneously.
Gateway Destination Vectors | Thermo Fisher Scientific - US
WEBGateway Destination Vectors. Versatile Gateway recombination cloning vectors. The Invitrogen Gateway Cloning Technology provides a rapid and highly efficient route to protein expression, functional analysis, and cloning/subcloning of DNA segments.
Gateway Cloning Protocols | Thermo Fisher Scientific - US
WEBThe Basics of Gateway cloning reactions. BP reaction—to create a Invitrogen Gateway entry clone; LR reaction—to create a Gateway expression clone; One tube format—to create a Gateway expression clone from a PCR product; Gateway vector conversion—converting your favorite cloning vectors to Gateway technology
Gateway® Cloning Vectors - SnapGene
WEBCommonly used Gateway® sequences including Donor Vectors, Entry Vectors, and Destination Vectors
Gateway and Golden Gate Vectors For Plant Transformation
WEBGateway technology by Invitrogen provides a quick method for cloning a DNA fragment into multiple expression vectors. At the VIB-Ugent Center for Plant Systems Biology, we have constructed over 200 versatile vectors for gene functional …
WEBThe Drosophila Gateway™ Vector collection is a set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies. At its core is Invitrogen's Gateway™ recombination cassette, which allows you to recombine an Open Reading Frame (ORF) of interest into any of the
WEBan Invitrogen™ Gateway™ vector, you can shuttle it to as many expression and functional analysis systems as you need. The diverse selection of expression vectors available with Gateway cloning technology is vast and broad. From expression proteins in E. coli, yeast, insect, or mammalian cells to RNAi